General Information
| Payroll Title | STAFF RESEARCH ASSOC IV | Title Code | 009610 | Grade/Step | Working Title | Operations Manager, Flow Cytometry | |
|---|---|---|---|---|---|---|---|
| Department Name | Institute of Genomics Dept | Supervisor | Glenn Hicks | Department Head | Natasha V Raikhel |
Special Requirements and Conditions
| Critical Position |
Level of Supervision Received
| General Direction |
Items Used
| ABI 3100 DNA analyzer |
| ABI 3730xl DNA sequencer |
| quantitative PCR instruments |
| centrifuges |
| FACS Aria flow cytometer |
| FACScan flow cytometer |
| PC/Mac Computers, Scanner, Fax Machine |
| PCR thermocyclers |
| Nanodrop 1000 |
| Affymetrix workstation |
| Illumina Cluster Station |
| Agilent Bioanalyzer |
| Illumina GAII DNA sequencer |
Position Purpose
| Independently operate, maintain and upgrade the fluorescent activated cell sorter (FACS) facility at the Institute for Integrative Genome Biology, providing highly-specialized expertise to researchers both on and off campus regarding advanced flow cytometry methodology. Consult with researchers from diverse disciplines including cell biology, biochemistry, biomedical and neurosciences, chemistry, environmental sciences, and plant biology on design of experimental procedures using FACS Aria and FACScan instrumentation and provide advice to customers on the significance of interpreted results and course of further experimentation. Additionally, manage gene expression analysis for IIGB clients and support genomics research across campus by providing DNA sequencing (Sanger and Ilumina) GeneChip services to more than 100 laboratories at UCR as well as to off-campus customers. Will also assist customers in the operations of other core lab instrumentation including qPCR, plate readers and imaging equipment. |
Essential Functions
| Essential Function | % Time |
|---|---|
| Independently operate and maintain the IIGB fluorescent activated cell sorter (FACS) facility. | 80 |
| Design experiments based on broadly outlined research goals of principal investigators, selecting methodology that best investigates scientific inquiries and directions. Interpret data, determine significance and impact to project, and provide further consultation to researchers on results and future directions. Conduct training for principal investigators and researchers regarding the field of flow cytometry, gene expression and the use of its instrumentation and associated software. Specific responsibilities include:
- Consult and collaborate with principal investigators on the nature and overall objectives of research projects and design experimental procedures based on operation of FACS Aria and FACScan instrumentation at Noel Keen Hall. Clients include UCR faculty, students and staff as well as other external campuses (i.e., Center for Health Disparities and Molecular Medicine at Loma Linda University and the USC/Keck School of Medicine, etc.). - Operate, maintain, repair and upgrade instrumentation. - Budget FACS facility operations and order all reagents and instrumentation necessary to maintain a state-of-the-art facility. - Perform complex statistical analyses which includes selecting appropriate analysis procedure, setting up data for analysis, judging validity and reliability of data, designing and writing programs to perform the analyses and producing written reports on the result of the analysis. - Communicate and discuss problems with the IIGB Academic Administrator. - Meet regularly with other genomics Core staff to coordinate FACS, gene expression and DNA sequencing operations. - Independently review professional literature to design appropriate research methodologies, techniques and procedures. - Keep current on cutting-edge, relevant techniques as dictated by interdisciplinary research projects of principal investigators. - Conduct training for principal investigators and other researchers in the use of software analysis tools including Diva, Cellquest-Pro, Modfit, and FlowJo. - Provide training in the use of the FACScan flow cytometer in Keen Hall. - Ensure that all environmental and health regulations are observed. |
|
| Independently perform gene expression analysis and fragment analysis for molecular mapping. | 10 |
| - Collaborate with PIs, postdocs and graduate students to design and implement strategies to investigate gene expression using Genechips.
- Ensure quality control of RNA samples, production of all labeled probes, and perform gene chip hybridization and gene expression analysis. - Oversee the training of a Research Assistant in Genechip technologies, DNA Sanger sequencing and fragment analysis. - Oversee a Research Assistant in providing services and collaboration in the area of Genechip technologies. - Perform all technical aspects of DNA fragment analysis within the Genomics Core facility. - Provide advice and technical leadership in DNA fragment analysis. |
|
| DNA Sequencing Services | 10 |
| - Assist laboratory workers in proper use of facilities and equipment.
- Operate and evaluate the quality of data from the newest generation of DNA sequencers, the Illumina HiSeq2000, within the IIGB. Provide assistance with DNA library construction, QC or analysis as needed. - Contribute to the broader UCR community by conducting tours of the Genomics and FACs facilities as part of IIGB's community outreach efforts and for the recruitment of new faculty and staff. |
|
Minimum Requirements
| Minimum Requirement | Display Ranking |
|---|---|
| 1 | |
| At least a BS in the biological sciences plus a minimum of 5 years of laboratory research or instrumentation facility experience, including 2 years in flow cytometry. | |
| 2 | |
| High-degree of technical expertise in the principles, operation, and maintenance of advanced flow cytometry instrumentation. | |
| 3 | |
| Ability to work independently under general supervision. | |
| 4 | |
| In depth knowledge of FACS instrumentation and ability to provide analytical services and collaborate with faculty, students and staff and external customers from diverse disciplines including biomedical and neurosciences, pathology, biochemistry, cell biology, and plant biology in experimental design, data interpretation and strategic planning of projects involving flow cytometry and gene expression. | |
| 5 | |
| Excellent oral and written communications skills and flexibility in dealing with clients from diverse scientific disciplines. | |
| 6 | |
| Proven ability to incorporate new instrumentation and methodologies in response to rapidly changing research needs. | |
| 7 | |
| Strong organizational skills; experience collecting and maintaining large volumes of data as well as records for services and billing. | |
| 8 | |
| Experience with and proficiency in additional areas of instrumentation and methodologies, including DNA sequencing using the ABI 3730xl sequencer and Affymetrix GeneChip approaches and instrumentation. The ability to work with clients and problem-solve in these areas is essential. Familiarity with the general principles of Illumina sequencing is required. | |
| 9 | |
| Proven ability to provide training (individually and in workshops) and provide guidance to principal investigators, students, postdocs and laboratory assistants in technical approaches and services provided by the Genomics Core. | |
| 10 | |
| Proficiency with software including flow cytometry analysis tools such as Diva, Cellquest-Pro, Modfit, and FlowJo in addition to Microsoft Office Suite. | |
Preferred Qualifications
| Preferred Qualification | Display Ranking |
|---|---|
| 1 | |
| Ability to make significant and original contributions to campus research projects in the fields of flow cytometry and gene expression. | |
| 2 | |
| Masters degree in the biological sciences including biochemistry, biology, genomics, plant biology and biomedical sciences. | |
| Posted Position Purpose |
|---|
| The Institute for Integrative Genome Biology (IIGB) is seeking an individual to Independently operate, maintain and upgrade the fluorescent activated cell sorter (FACS) within their genomics core instrumentation facility. The innovative and multidisciplinary IIGB is organized around a 10,000 sq.ft. suite of instrumentation facilities that serve as a centralized, shared-use resource for faculty, staff, students, offering advanced tools in bioinformatics, microscopy and imaging, proteomics and genomics. The position will be responsible for providing highly-specialized expertise to on- and off-campus researchers from diverse disciplines including cell biology, biochemistry, biomedical and neurosciences, chemistry, environmental sciences, and plant biology regarding advanced flow cytometry methodology, consulting on the design of experimental procedures using FACS Aria and FACScan instrumentation, and advising on the significance of interpreted results and the course of further experimentation. In addition, the position will manage gene expression analysis for IIGB clients and support genomics research across campus by providing DNA sequencing (Sanger and Ilumina) GeneChip services to more than 100 laboratories at UCR as well as to off-campus customers. The position will also assist customers in the operation of other core lab instrumentation including qPCR, plate readers and imaging equipment .
|
| Posted Minimum Requirements |
| 1. At least a BS in the biological sciences plus a minimum of 5 years of laboratory research or instrumentation facility experience, including 2 years in flow cytometry.
2. High-degree of technical expertise in the principles, operation, and maintenance of advanced flow cytometry instrumentation. 3. Ability to work independently under general supervision. 4. In depth knowledge of FACS instrumentation and ability to provide analytical services and collaborate with faculty, students and staff and external customers from diverse disciplines including biomedical and neurosciences, pathology, biochemistry, cell biology, and plant biology in experimental design, data interpretation and strategic planning of projects involving flow cytometry and gene expression. 5. Excellent oral and written communications skills and flexibility in dealing with clients from diverse scientific disciplines. 6. Proven ability to incorporate new instrumentation and methodologies in response to rapidly changing research needs. 7. Strong organizational skills; experience collecting and maintaining large volumes of data as well as records for services and billing. 8. Experience with and proficiency in additional areas of instrumentation and methodologies, including DNA sequencing using the ABI 3730xl sequencer and Affymetrix GeneChip approaches and instrumentation. The ability to work with clients and problem-solve in these areas is essential. Familiarity with the general principles of Illumina sequencing is required. 9. Proven ability to provide training (individually and in workshops) and provide guidance to principal investigators, students, postdocs and laboratory assistants in technical approaches and services provided by the Genomics Core. 10. Proficiency with software including flow cytometry analysis tools such as Diva, Cellquest-Pro, Modfit, and FlowJo in addition to Microsoft Office Suite. |
| Posted Preferred Qualifications |
| 1. Ability to make significant and original contributions to campus research projects in the fields of flow cytometry and gene expression.
2. Masters degree in the biological sciences including biochemistry, biology, genomics, plant biology and biomedical sciences |