General Information
| Payroll Title | LAB AST 2 | Title Code | 009603 | Grade/Step | Working Title | Lab Assistant | |
|---|---|---|---|---|---|---|---|
| Department Name | Biomedical Sciences Dept | Supervisor | Sika Zheng | Department Head | Monica J Carson |
Special Requirements and Conditions
| Critical Position |
| Overtime |
Level of Supervision Received
| General Supervision |
Items Used
| DNA and RNA extraction and purification |
| Standard lab equipment |
| Standard office equipment |
Position Purpose
| Under supervision, perform one or more standard repetitive laboratory procedures that require technical scientific knowledge, following all safety standards in a teaching or research laboratory. Assist PI and/or lab members with the execution of various laboratory procedures and the performance of ongoing associated research projects in the Zheng lab. The responsibilities include maintaining mouse breeding colonies and lab-generated inventories (DNA plasmids and cell lines) and lab chores. Other responsibilities may include sectioning, cloning, cell culture, and other duties as research needs fluctuate or change. The applicant should be comfortable with handling, breeding & genotyping of animals, including timed matings. |
Essential Functions
| Essential Function | % Time |
|---|---|
| Animal colony maintenance and assisting with experiments | 70 |
| Organize and keep laboratory animal breeding colony. Keep track of the mouse colony and write Animal Use Protocol/Forms for future experiments conducted by lab members. Occasionally assist with postdocs/students with lab animal experimental colony. Assist with data analysis and database updates as needed for the operations of the laboratory. Assist with laboratory experiments using a variety of genetic, molecular and biochemical approaches, incl. DNA and RNA extraction, cloning, and PCR/RT-PCR. Handling, breeding and genotyping of animals. Harvest organs from animals and perform sectioning. Maintain, freeze, thaw cell lines. |
|
| Data collection and record keeping | 15 |
| Assist in data collection and accurate record keeping. Assist with University laboratory safety audits. |
|
| Laboratory maintenance | 15 |
| General laboratory duties: Preparation of basic solutions and reagents. Assist in keeping a clean and safe working environment in accord with Environmental Health and Safety guidelines. Maintain, order and store laboratory supplies and equipment. |
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Minimum Requirements
| Minimum Requirement | Display Ranking |
|---|---|
| No information record yet | |
Preferred Qualifications
| Preferred Qualification | Display Ranking |
|---|---|
| No information record yet | |
| Posted Position Purpose |
|---|
| Under supervision, perform one or more standard repetitive laboratory procedures that require technical scientific knowledge, following all safety standards in a teaching or research laboratory. Assist PI and/or lab members with the execution of various laboratory procedures and the performance of ongoing associated research projects in the Zheng lab. The responsibilities include maintaining mouse breeding colonies and lab-generated inventories (DNA plasmids and cell lines) and lab chores. Other responsibilities may include sectioning, cloning, cell culture, and other duties as research needs fluctuate or change. The applicant should be comfortable with handling, breeding & genotyping of animals, including timed matings. |
| Posted Minimum Requirements |
| 1. Knowledge of use of routine lab equipment. 2. College-level coursework in biological science, chemistry, physics or related area. 3. Ability to read, write, and perform basic mathematical calculations. 4. Ability to follow oral and written instructions. 5. Demonstrated computer proficiency. 6. Skill in animal handling, in particular in vivo procedures, including but not limited to, timed matings. 7. Minimum of 2 years related laboratory experience. 8. Ability to follow laboratory protocols and work independently after initial supervision. 9. Ability to multi-task, adapt to changing work priorities and workloads. 10. Experience with cell culture, RNA extraction and RT-PCR, Western blotting. |
| Posted Preferred Qualifications |
| 1. Experience in handling transgenic and knockout rodents. 2. Bachelor's degree in biological science, chemistry, physics or related area. 3. Ability to perform or willingness to learn stereotactic injections. |